Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots

0497527 - ÚIACH 2019 RIV CZ eng C - Conference Paper (international conference)
Dvořák, Miloš - Miková, B. - Šlampová, Andrea - Kubáň, Pavel
Hollow fibre-liquid phase microextraction of basic drugs from dried dried blood spots.
CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis. Brno: Ústav analytické chemie AV ČR, v. v. i., 2018 - (Foret, F.; Křenková, J.; Drobníková, I.; Klepárník, K.; Přikryl, J.), s. 52-54. ISBN 978-80-904959-5-1.
[CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./. Brno (CZ), 15.10.2018-17.10.2018]
R&D Projects: GA ČR(CZ) GA18-13135S; GA ČR(CZ) GA16-09135S
Institutional support: RVO:68081715
Keywords : hollow fibre-liquid phase microextraction * microextraction of basic drugs * dried blood spots
OECD category: Analytical chemistry
http://www.ce-ce.org/user_uploads/program/CECE%202018%20Proceedings_WOS.pdf

Hollow fibre-liquid phase microextraction (HF-LPME) was applied for rapid and efficient extraction of basic drugs from dried blood spots (DBS). Basic drugs from DBS were first extracted into 10 mM NaOH solution, which acted as the HF-LPME donor solution, and simultaneously the neutralized drugs were transferred across the HF and preconcentrated in 10 mM HCl acceptor solution inside the HF lumen. Mixture (1:1, v:v) of 1-ethyl-2-nitrobenzene and dihexyl ether was used for impregnation of the porous HF membrane. Analytes in the acceptor solution were injected directly from the HF into capillary electrophoresis instrument for further separation, detection and quantification. Repeatability of the hyphenated analytical system ranged from 9.8 to 13.8 % (RSD) and enrichment factors of 52 – 78 were obtained for model basic drugs spiked to DBS extracts at a concentration level of 0.1 mg/L.
Permanent Link: http://hdl.handle.net/11104/0290091