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Chromosome-based survey sequencing reveals the genome organization of wild wheat progenitor Triticum dicoccoides
- 1.0497342 - ÚEB 2019 RIV US eng J - Journal Article
Akpinar, B. A. - Biyiklioglu, S. - Alptekin, B. - Havránková, Miroslava - Vrána, Jan - Doležel, Jaroslav - Distelfeld, A. - Hernandez, P. - Budak, H.
Chromosome-based survey sequencing reveals the genome organization of wild wheat progenitor Triticum dicoccoides.
Plant Biotechnology Journal. Roč. 16, č. 12 (2018), s. 2077-2087. ISSN 1467-7644. E-ISSN 1467-7652
R&D Projects: GA ČR GBP501/12/G090; GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : chromosome sorting * comparative genomics * hexaploid wheat * next-generation sequencing * wild emmer wheat
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 6.840, year: 2018
Wild emmer wheat (Triticum turgidum ssp. dicoccoides) is the progenitor of wheat. We performed chromosome-based survey sequencing of the 14 chromosomes, examining repetitive sequences, protein-coding genes, miRNA/target pairs and tRNA genes, as well as syntenic relationships with related grasses. We found considerable differences in the content and distribution of repetitive sequences between the A and B subgenomes. The gene contents of individual chromosomes varied widely, not necessarily correlating with chromosome size. We catalogued candidate agronomically important loci, along with new alleles and flanking sequences that can be used to design exome sequencing. Syntenic relationships and virtual gene orders revealed several small-scale evolutionary rearrangements, in addition to providing evidence for the 4AL-5AL-7BS translocation in wild emmer wheat. Chromosome-based sequence assemblies contained five novel miRNA families, among 59 families putatively encoded in the entire genome which provide insight into the domestication of wheat and an overview of the genome content and organization.
Permanent Link: http://hdl.handle.net/11104/0289905
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