Distinct communities of poplar endophytes on an unpolluted and a risk elements-polluted site and their plant growth promoting potential in vitro

0497079 - BÚ 2019 RIV US eng J - Journal Article
Schmidt, Christoph Stephan - Lovecká, P. - Mrnka, Libor - Vychodilová, I. - Strejček, M. - Fenclová, M. - Demnerová, K.
Distinct communities of poplar endophytes on an unpolluted and a risk elements-polluted site and their plant growth promoting potential in vitro.
Microbial Ecology. Roč. 75, č. 4 (2018), s. 955-969. ISSN 0095-3628. E-ISSN 1432-184X
R&D Projects: GA TA ČR TA03011184
Institutional support: RVO:67985939
Keywords : hybrid poplar * endophytic bacteria and fungi * in vitro tests
OECD category: Microbiology
Impact factor: 3.611, year: 2018

Numerous studies demonstrated that endophytic microbes can promote plant growth and increase plant stress resistance. We aimed at isolating poplar endophytes able to increase their hosts' fitness both in nutrients-limited and polluted environments. To achieve the goal endophytic bacteria and fungi were isolated from roots and leaves of hybrid poplars (Populus nigra × P. maximowiczii clone Max-4) on an unpolluted and a risk elements-polluted site in the Czech Republic and subsequently screened by a number of in vitro tests. Bacterial communities at the unpolluted site were dominated by gammaproteobacteria with Pseudomonas sp. as the prominent member of the class, followed by Bacilli with prevailing Bacillus sp., whereas alphaproteobacteria, mostly Rhizobium sp., prevailed at the polluted site. The fungal endophytic community was dominated by ascomycetes and highly distinct on both sites. Dothideomycetes, mostly Cladosporium, prevailed at the non-polluted site while unclassified Sordariomycetous fungi dominated at the polluted site. Species diversity of endophytes was higher at the unpolluted site. Many tested endophytic strains solubilized phosphate and produced siderophores, phytohormones and antioxidants. Some strains also exhibited ACC-deaminase activity. Selected bacteria showed high tolerance and the ability to accumulate risk elements, making them promising candidates for use in inocula promoting biomass production and phytoremediation.
Permanent Link: http://hdl.handle.net/11104/0289691