An optimized FAIRE procedure for low cell numbers in yeast

0497014 - MBÚ 2019 RIV GB eng J - Journal Article
Segorbe, D. - Wilkinson, D. - Mizeranschi, A. - Hughes, T. - Aalokken, R. - Váchová, Libuše - Palková, Z. - Gilfillan, G.D.
An optimized FAIRE procedure for low cell numbers in yeast.
Yeast. Roč. 35, č. 8 (2018), s. 507-512. ISSN 0749-503X. E-ISSN 1097-0061
R&D Projects: GA MŠMT(CZ) 7F14083; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:61388971
Keywords : chromatin-accessibility * epigenetics * HTS
OECD category: Microbiology
Impact factor: 2.395, year: 2018

We report an optimized low-input FAIRE-seq (Formaldehyde-Assisted Isolation of Regulatory Elements-sequencing) procedure to assay chromatin accessibility from limited amounts of yeast cells. We demonstrate that the method performs well on as little as 4mg of cells scraped directly from a few colonies. Sensitivity, specificity and reproducibility of the scaled-down method are comparable with those of regular, higher input amounts, and allow the use of 100-fold fewer cells than existing procedures. The method enables epigenetic analysis of chromatin structure without the need for cell multiplication of exponentially growing cells in liquid culture, thus opening the possibility of studying colony cell subpopulations, or those that can be isolated directly from environmental samples.
Permanent Link: http://hdl.handle.net/11104/0289625