Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan

0496977 - BTÚ 2019 RIV GB eng J - Journal Article
Šindelka, Radek - Abaffy, Pavel - Qu, Y. - Tománková, Silvie - Šídová, Monika - Naraine, Ravindra - Kolář, Michal - Peuchen, E. - Sun, L. - Dovichi, N. - Kubista, Mikael
Asymmetric distribution of biomolecules of maternal origin in the Xenopus laevis egg and their impact on the developmental plan.
Scientific Reports. Roč. 8, MAY 29 2018 (2018), č. článku 8315. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA16-07500S; GA MŠMT(CZ) LH15074; GA MŠMT(CZ) LM2015047; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036 ; RVO:68378050
Keywords : CELL MASS-SPECTROMETRY * BICOID MESSENGER-RNA * SAMPLE PREPARATION
OECD category: Other biological topics; Other biological topics (UMG-J)
Impact factor: 4.011, year: 2018

Asymmetric cell division is a ubiquitous feature during the development of higher organisms. Asymmetry is achieved by differential localization or activities of biological molecules such as proteins, and coding and non-coding RNAs. Here, we present subcellular transcriptomic and proteomic analyses along the animal-vegetal axis of Xenopus laevis eggs. More than 98% of the maternal mRNAs could be categorized into four localization profile groups: animal, vegetal, extremely vegetal, and a newly described group of mRNAs that we call extremely animal, which are mRNAs enriched in the animal cortex region. 3'UTRs of localized mRNAs were analyzed for localization motifs. Several putative motifs were discovered for vegetal and extremely vegetal mRNAs, while no distinct conserved motifs for the extremely animal mRNAs were identified, suggesting different localization mechanisms. Asymmetric profiles were also found for proteins, with correlation to those of corresponding mRNAs. Based on unexpected observation of the profiles of the homoeologous genes exd2 we propose a possible mechanism of genetic evolution.
Permanent Link: http://hdl.handle.net/11104/0289597