Diamond nanoparticles suppress lateral growth of bacterial colonies

0496974 - FZÚ 2019 RIV NL eng J - Journal Article
Lišková, P. - Beranová, J. - Ukraintsev, Egor - Fišer, R. - Kofroňová, Olga - Benada, Oldřich - Konopásek, I. - Kromka, Alexander
Diamond nanoparticles suppress lateral growth of bacterial colonies.
Colloids and Surfaces B-Biointerfaces. Roč. 170, Oct (2018), s. 544-552. ISSN 0927-7765. E-ISSN 1873-4367
R&D Projects: GA ČR GA15-01687S; GA MŠMT(CZ) LO1509
Institutional support: RVO:68378271 ; RVO:61388971
Keywords : agar surface * bacteria * colony growth * diamond nanoparticles
OECD category: Microbiology; Analytical chemistry (MBU-M)
Impact factor: 3.973, year: 2018

Diamond nanoparticles (DNPs) of various types have been recently reported to possess antibacterial properties. Studies have shown a decrease of the colony forming ability on agar plates of the bacteria that had been previously co-incubated with DNPs in the suspension. The effect of DNPs remaining on the surface, alongside growing bacteria, has not been previously investigated. We present the experiments designed to investigate the effect of DNPs on bacterial survival and on the growth of the bacterial colony on a solid media. We employed Escherichia coli and Bacillus subtilis as models of Gram-negative and Gram-positive bacteria, respectively, and Proteus mirabilis as a model of bacterium exhibiting swarming motility on the surfaces. We analyzed the number, area, and weight of bacterial colonies grown on the agar surface covered with DNPs. We did not observe any bactericidal effect of such applied DNPs. We show that DNPs act as the mechanical barrier blocking the lateral colony growth.

Permanent Link: http://hdl.handle.net/11104/0289616