TATAA courses – 2 Days qPCR for miRNA analysis\n\n

0496888 - BTÚ 2019 RIV eng U - Conference, Workshop Arrangement
Kubista, Mikael - Valihrach, Lukáš - Androvič, Peter - Rohlová, Eva
TATAA courses – 2 Days qPCR for miRNA analysis

.
[Vestec, BIOCEV, 10.05.2018-11.05.2018, (W-WRD 11/5)]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : qPCR * quality control * miRNA analysis
OECD category: Biochemistry and molecular biology

This course introduces miRNA and its variant forms. Methods to extract and purify microRNA and prepare it for analysis, including quality control. Methods for microRNA expression analysis are presented and compared, to learn how to too choose the best method for each study. Analysis of microRNA data is also discussed, including challenging normalization. The course contains:
Day 1
• MicroRNA background including biogenesis and function
• MicroRNA extraction – sampling, miRNA stability, extraction kit comparison
• Quality Control of miRNA
• MicroRNA and liquid biopsies – challenges of miRNA analysis in biofluids
• Hands-on lab testing of quality and quantity of miRNA
Day 2
• MicroRNA quantification with microarray, nanoString, NGS and RT -qPCR methods
• Comparison of miRNA quantification methods
• Experimental design and data analysis
• RT-qPCR normalization of miRNA data
• Introduction to NGS data analysis
• Hands-on lab of miRNA RT-qPCR analysis including data analysis in Genex

Permanent Link: http://hdl.handle.net/11104/0289503