Growth of Primary Human Osteoblasts on Plasma-Treated and Nanodiamond-Coated PTFE Polymer Foils

0496185 - FGÚ 2019 RIV DE eng J - Journal Article
Kopová, Ivana - Rezek, Bohuslav - Stehlík, Štěpán - Ukraintsev, Egor - Kasálková-Slepičková, N. - Slepička, P. - Potocký, Štěpán - Bačáková, Lucie
Growth of Primary Human Osteoblasts on Plasma-Treated and Nanodiamond-Coated PTFE Polymer Foils.
Physica Status Solidi B. Roč. 255, č. 10 (2018), č. článku 1700595. ISSN 0370-1972. E-ISSN 1521-3951
R&D Projects: GA MZd(CZ) NV15-32497A
Institutional support: RVO:67985823 ; RVO:68378271
Keywords : nanodiamonds * osteoblasts * plasma treatment * proliferation * wettability
OECD category: Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials; Biophysics (FZU-D)
Impact factor: 1.454, year: 2018

Polytetrafluoroethylene (PTFE) is widely used for constructing tissue replacements, particularly clinically used vascular prostheses, and is also applied in dental and orthopedic surgery, thanks to its non‐toxicity, high chemical resistance, low surface energy and excellent thermal stability. We report here on a comparative study in which PTFE is modified with the use of DC argon plasma (8 W, 240 s) and is coated with hydrogenated or oxidized nanodiamonds (mean size 5 nm), with a view to achieving improved body acceptance of the bio‐inert pristine material. The surface morphology characterized by scanning electron microscopy reveals a microscale and nanoscale structuring of the PTFE foils with comparable roughness among all samples (analyzed by atomic force microscopy). The water contact angle remains in the highly hydrophobic range (above 100°). However, the proliferation and metabolic activity of primary human hFOB 1.19 osteoblasts (studied for up to 7 days) are significantly enhanced by the plasma and/or by hydrogenated nanodiamond treatment (rather than by oxidized nanodiamond treatment) of the PTFE foil.
Permanent Link: http://hdl.handle.net/11104/0289009