Insect fat body cell morphology and response to cold stress is modulated by acclimation.

0495783 - BC 2019 RIV GB eng J - Journal Article
Des Marteaux, Lauren E. - Štětina, Tomáš - Košťál, Vladimír … Total 8 authors
Insect fat body cell morphology and response to cold stress is modulated by acclimation.
Journal of Experimental Biology. Roč. 221, č. 21 (2018), č. článku jeb189647. ISSN 0022-0949. E-ISSN 1477-9145
R&D Projects: GA ČR(CZ) GA16-06374S
Institutional support: RVO:60077344
Keywords : freeze tolerance * Chymomyza * diapause
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 3.017, year: 2018
http://jeb.biologists.org/content/early/2018/09/05/jeb.189647

We exploited the broadly-manipulable freeze tolerance of larval malt flies (Chymomyza costata) to uncover cell and tissue morphological changes associated with freeze mortality. Using confocal microscopy and immunostaining of the fat body, Malpighian tubules, and anterior midgut we described tissue and cytoskeletal (F-actin and α-tubulin) morphologies among the acclimation variants after exposure to various cold stresses (from chilling at -5ºC to extreme freezing at -196ºC), and upon recovery from cold exposure. Our observations
indicate that lipid coalescence and damage to α-tubulin are non-lethal forms of freeze injury, and suggest that repair or removal (rather than protection) of actin proteins is a potential mechanism of acquired freeze tolerance.
Permanent Link: http://hdl.handle.net/11104/0292235