Characterization and annotation of oxidized glycerophosphocholines for non-targeted metabolomics with LC-QTOF-MS data

0495424 - ÚEB 2019 RIV NL eng J - Journal Article
de la Fuente, A.G. - Traldi, F. - Široká, Jitka - Kretowski, A. - Ciborowski, M. - Otero, A. - Barbas, C. - Godzien, J.
Characterization and annotation of oxidized glycerophosphocholines for non-targeted metabolomics with LC-QTOF-MS data.
Analytica Chimica Acta. Roč. 1037, DEC 11 (2018), s. 358-368. ISSN 0003-2670. E-ISSN 1873-4324
R&D Projects: GA MŠMT(CZ) LO1204
Institutional support: RVO:61389030
Keywords : mass-spectrometry * phospholipid oxidation * identification * inflammation * products * cells * hdl * Non-targeted metabolomics * Identification * Annotation * Oxidized glycerophosphocholines * oxPAPC * lc-esi-qtof
OECD category: Cell biology
Impact factor: 5.256, year: 2018

The biological role of oxidized glycerophosphocholines (oxPCs) is a current topic of research importantly contributing to the understanding of health and disease. Global non-targeted metabolomics offers an interesting approach to expand current knowledge and link oxPCs to new biological functions. Although this strategy is successful, it also has some limitations which are clearly noticeable during the identification process. For this reason, clear rules related to the identification of each group of metabolites are needed. This work attempts to provide the reader with a guideline for the recognition and annotation of oxidation among phosphocholines (PCs). Using several chromatographic characteristics and spectral information from tandem mass spectrometry, rapid and reliable annotation of long and short chain oxPCs can be performed. Some of this knowledge has been implemented in the publicly available annotation tool 'CEU Mass Mediator' (CMM) for semi-automated assignment of oxidation. Additionally, this tool was supplemented with accurate monoisotopic masses of oxPCs, expanding current information in other databases. Moreover, the characterization of oxidization products of PC(16:0/20:4) known as PAPC has been performed, providing a list of accurate mass product ions and neutral losses.
Permanent Link: http://hdl.handle.net/11104/0288414