The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes

0495317 - ÚEB 2019 RIV US eng J - Journal Article
Said, Mahmoud - Hřibová, Eva - Danilova, T. - Karafiátová, Miroslava - Čížková, Jana - Friebe, B. - Doležel, Jaroslav - Gill, B.S. - Vrána, Jan
The Agropyron cristatum karyotype, chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes.
Theoretical and Applied Genetics. Roč. 131, č. 10 (2018), s. 2213-2227. ISSN 0040-5752. E-ISSN 1432-2242
R&D Projects: GA ČR(CZ) GA16-16992S
Institutional support: RVO:61389030
Keywords : repetitive dna-sequences * barley yellow dwarf * diploid crested wheatgrasses * carrying rust resistance * rna multigene family * rye secale-cereale * common wheat * triticum-aestivum * cytogenetic characterization * conferring resistance
OECD category: Genetics and heredity (medical genetics to be 3)
Impact factor: 3.926, year: 2018

Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem repeats revealed homoeologous relationships of Agropyron cristatum with wheat. The results will contribute to alien gene introgression in wheat improvement.
Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat and a promising source of novel genes for wheat improvement. To date, identification of A. cristatum chromosomes has not been possible, and its molecular karyotype has not been available. Furthermore, homoeologous relationship between the genomes of A. cristatum and wheat has not been determined. To develop chromosome-specific landmarks, A. cristatum genomic DNA was sequenced, and new tandem repeats were discovered. Their distribution on mitotic chromosomes was studied by fluorescence in situ hybridization (FISH), which revealed specific patterns for five repeats in addition to 5S and 45S ribosomal DNA and rye subtelomeric repeats pSc119.2 and pSc200. FISH with one tandem repeat together with 45S rDNA enabled identification of all A. cristatum chromosomes. To analyze the structure and cross-species homoeology of A. cristatum chromosomes with wheat, probes for 45 mapped wheat cDNAs covering all seven chromosome groups were localized by FISH. Thirty-four cDNAs hybridized to homoeologous chromosomes of A. cristatum, nine hybridized to homoeologous and non-homoeologous chromosomes, and two hybridized to unique positions on non-homoeologous chromosomes. FISH using single-gene probes revealed that the wheat-A. cristatum collinearity was distorted, and important structural rearrangements were observed for chromosomes 2P, 4P, 5P, 6P and 7P. Chromosomal inversions were found for pericentric region of 4P and whole chromosome arm 6PL. Furthermore, reciprocal translocations between 2PS and 4PL were detected. These results provide new insights into the genome evolution within Triticeae and will facilitate the use of crested wheatgrass in alien gene introgression into wheat.
Permanent Link: http://hdl.handle.net/11104/0288311