Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa)

0495283 - ÚPT 2019 RIV DE eng J - Journal Article
Kováčiková, M. - Vaškovicová, Naděžda - Nebesářová, Jana - Valigurová, A.
Effect of jasplakinolide and cytochalasin D on cortical elements involved in the gliding motility of the eugregarine Gregarina garnhami (Apicomplexa).
European Journal of Protistology. Roč. 66, OCT (2018), s. 97-114. ISSN 0932-4739. E-ISSN 1618-0429
R&D Projects: GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01; GA MŠMT(CZ) LM2015062
Institutional support: RVO:68081731 ; RVO:60077344
Keywords : ectoplasmic network * F-actin * Gregarine * motility * myonemes * ultrastructure
OECD category: Cell biology; Genetics and heredity (medical genetics to be 3) (BC-A)
Impact factor: 2.626, year: 2018

Since apicomplexans represent exclusively parasitic unicellular organisms with medical and economic impacts, the principles of their motility have been studied intensively. By contrast, the movement in apicomplexan basal groups, such as gregarines, remains to be elucidated. The present study focuses on Gregarina garnhami parasitising the digestive tract of the locust Schistocerca gregaria, and investigates the involvement of cytoskeletal elements (the ectoplasmic network and myonemes) and the secretion of mucosubstances during eugregarine gliding motility. Combined microscopic analyses were used to verify the role of actin filaments and membranes’ organisation in G. garnhami motility. A freeze-etching analysis of membranes revealed the size, density, and arrangement of intramembranous particles along with the distribution and size of pores and ducts. Experimental assays using actin-modifying drugs (jasplakinolide, cytochalasin D) confirmed that actin most likely plays a role in cell motility, principally in its filamentous form (=F-actin). Myonemes, localised in the border between the ectoplasm and endoplasm, correspond to the concentric bundles of F-actin. Microscopic analyses confirmed that changes in gamonts motility corresponding to the changes in the organisation and density of myonemes and the ectoplasmic network in drug-treated cells, suggesting that these structures might serve as contractile elements facilitating gliding motility in G. garnhami.
Permanent Link: http://hdl.handle.net/11104/0288280