Stabilization of hyaluronan-based materials by peptide conjugation and its use as a cell-seeded scaffold in tissue engineering

0495240 - ÚOCHB 2019 RIV GB eng J - Journal Article
Karel, S. - Sogorková, J. - Hermannová, M. - Nešporová, K. - Marholdová, L. - Chmelíčková, K. - Bednárová, Lucie - Flegel, M. - Drašar, P. - Velebný, V.
Stabilization of hyaluronan-based materials by peptide conjugation and its use as a cell-seeded scaffold in tissue engineering.
Carbohydrate Polymers. Roč. 201, Dec 1 (2018), s. 300-307. ISSN 0144-8617. E-ISSN 1879-1344
Institutional support: RVO:61388963
Keywords : hyaluronan * SPPS * fibers * non-woven textiles * RGD * cell adhesion
OECD category: Polymer science
Impact factor: 6.044, year: 2018

New materials based on molecules naturally occurred in body are assumed to be fully bio-compatible and biodegradable. In our study, we used hyaluronic acid (HA) modified with peptides, which meet all this criterion and could be advantageously used in tissue engineering. Peptides with RGD, IKVAV or SIKVAV adhesive motif were attached to HA-based fiber or non-woven textile through ester bond in the term of solid phase peptide synthesis. A linker between HA and peptide containing three glycine or two 6-aminohexanoyl units was applied to make peptides more available for cell surface receptors. Dermal fibroblasts adhered readily on this material, preferentially to RGD peptide with 6-aminohexanoyl linker. Contrary, the absence of adhesive peptide did not allow the cell attachment but maintained the material stability.
Permanent Link: http://hdl.handle.net/11104/0288245