Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing

0494894 - ÚIACH 2019 RIV GB eng J - Journal Article
Chen, W. - Duša, Filip - Witos, J. - Ruokonen, S. K. - Wiedmer, S. K.
Determination of the main phase transition temperature of phospholipids by nanoplasmonic sensing.
Scientific Reports. Roč. 8, OCT (2018), č. článku 14815. ISSN 2045-2322. E-ISSN 2045-2322
Grant - others:AV ČR(CZ) MSM200311602
Program: Program na podporu mezinárodní spolupráce začínajících výzkumných pracovníků
Institutional support: RVO:68081715
Keywords : drug-delivery systems * ionic liquids * nanomaterials science * biological-membranes * liposome
OECD category: Nano-processes (applications on nano-scale)
Impact factor: 4.011, year: 2018

Our study demonstrates that nanoplasmonic sensing (NPS) can be utilized for the determination of the phase transition temperature (T-m) of phospholipids. During the phase transition, the lipid bilayer undergoes a conformational change. Therefore, it is presumed that the T-m of phospholipids can be determined by detecting conformational changes in liposomes. The studied lipids included 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC), and 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC). Liposomes in gel phase are immobilized onto silicon dioxide sensors and the sensor cell temperature is increased until passing the T-m of the lipid. The results show that, when the system temperature approaches the T-m, a drop of the NPS signal is observed. The breakpoints in the temperatures are 22.5 degrees C, 41.0 degrees C, and 55.5 degrees C for DMPC, DPPC, and DSPC, respectively. These values are very close to the theoretical T-m values, i.e., 24 degrees C, 41.4 degrees C, and 55 degrees C for DMPC, DPPC, and DSPC, respectively. Our studies prove that the NPS methodology is a simple and valuable tool for the determination of the T-m of phospholipids.
Permanent Link: http://hdl.handle.net/11104/0287940