Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription

0494648 - ÚMG 2019 RIV GB eng J - Journal Article
Sobol, Margaryta - Krausová, Alžběta - Yildirim, Sukriye - Kalasová, Ilona - Fáberová, Veronika - Vrkoslav, Vladimír - Philimonenko, Vlada - Marášek, Pavel - Pastorek, Lukáš - Čapek, Martin - Lubovská, Zuzana - Uličná, Lívia - Tsuji, T. - Lísa, M. - Cvačka, Josef - Fujimoto, T. - Hozák, Pavel
Nuclear phosphatidylinositol 4,5-bisphosphate islets contribute to efficient RNA polymerase II-dependent transcription.
Journal of Cell Science. Roč. 131, č. 8 (2018), č. článku jcs211094. ISSN 0021-9533. E-ISSN 1477-9137
R&D Projects: GA ČR GAP305/11/2232; GA ČR(CZ) GA16-03346S; GA ČR GA15-08738S; GA TA ČR(CZ) TE01020118; GA MŠMT(CZ) EF16_013/0001775; GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:68378050 ; RVO:61388963
Keywords : Nucleus * PtdIns(4,5)P-2 * DNA transcription * Chromatin * RNA polymerase II * Phosphoinositides
OECD category: Cell biology; Analytical chemistry (UOCHB-X)
Impact factor: 4.517, year: 2018

This paper describes a novel type of nuclear structure nuclear lipid islets (NLIs). They are of 40-100 nm with a lipidic interior, and phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P-2] molecules comprise a significant part of their surface. Most of NLIs have RNA at the periphery. Consistent with that, RNA is required for their integrity. The NLI periphery is associated with Pol II transcription machinery, including the largest Pol II subunit, transcription factors and NM1 (also known as NMI). The Ptdlns(4,5)P-2-NM1 interaction is important for Pol II transcription, since NM1 knockdown reduces the Pol II transcription level, and the overexpression of wild-type NM1 [but not NM1 mutated in the Ptdlns(4,5)P-2-binding site] rescues the transcription. Importantly, Pol II transcription is dependent on NLI integrity, because an enzymatic reduction of the Ptdlns(4,5)P-2 level results in a decrease of the Pol II transcription level. Furthermore, about half of nascent transcripts localise to NLIs, and transcriptionally active transgene loci preferentially colocalise with NLIs. We hypothesize that NLIs serve as a structural platform that facilitates the formation of Pol II transcription factories, thus participating in the formation of nuclear architecture competent for transcription.
Permanent Link: http://hdl.handle.net/11104/0287754