Low temperature induced modulation of photosynthetic induction in non-acclimated and cold-acclimated Arabidopsis thaliana: chlorophyll a fluorescence and gas-exchange measurements

0494442 - ÚVGZ 2020 RIV NL eng J - Journal Article
Mishra, Kumud - Mishra, Anamika - Urban, Otmar - Kubásek, Jiří - Heyer, A. G. - Govindjee, G.
Low temperature induced modulation of photosynthetic induction in non-acclimated and cold-acclimated Arabidopsis thaliana: chlorophyll a fluorescence and gas-exchange measurements.
Photosynthesis Research. Roč. 139, 1-3 (2019), s. 123-143. ISSN 0166-8595. E-ISSN 1573-5079
R&D Projects: GA MŠMT(CZ) LO1415; GA MŠMT(CZ) EF16_013/0001609
Research Infrastructure: CzeCOS II - 90061
Institutional support: RVO:86652079
Keywords : Low-temperature effect * Cold acclimation * Chlorophyll fluorescence transients * low SMT fluorescences phase * Gas-exchange measurements * State transition 3-(3, 4-dichlorophenyl)-1, 1- dimethylurea * Methyl viologen
OECD category: Plant sciences, botany
Impact factor: 3.216, year: 2019
Method of publishing: Open access
https://link.springer.com/article/10.1007%2Fs11120-018-0588-7

Cold acclimation modifies the photosynthetic machinery and enables plants to survive at sub-zero temperatures, whereas in warm habitats, many species suffer even at non-freezing temperatures. We have measured chlorophyll a fluorescence (ChlF) and CO2 assimilation to investigate the effects of cold acclimation, and of low temperatures, on a cold-sensitive Arabidopsis thaliana accession C24. Upon excitation with low intensity (40 µmol photons m− 2 s− 1) ~ 620 nm light, slow (minute range) ChlF transients, at ~ 22 °C, showed two waves in the SMT phase (S, semi steady-state, M, maximum, T, terminal steady-state), whereas CO2 assimilation showed a linear increase with time. Low-temperature treatment (down to − 1.5 °C) strongly modulated the SMT phase and stimulated a peak in the CO2 assimilation induction curve. We show that the SMT phase, at ~ 22 °C, was abolished when measured under high actinic irradiance, or when 3-(3, 4-dichlorophenyl)-1, 1- dimethylurea (DCMU, an inhibitor of electron flow) or methyl viologen (MV, a Photosystem I (PSI) electron acceptor) was added to the system. Our data suggest that stimulation of the SMT wave, at low temperatures, has multiple reasons, which may include changes in both photochemical and biochemical reactions leading to modulations in non-photochemical quenching (NPQ) of the excited state of Chl, “state transitions,” as well as changes in the rate of cyclic electron flow through PSI. Further, we suggest that cold acclimation, in accession C24, promotes “state transition” and protects photosystems by preventing high excitation pressure during low-temperature exposure.
Permanent Link: http://hdl.handle.net/11104/0289179