Thickness determination of a cathodoluminescence active nanoparticles by means of Quantitative STEM imaging

0494375 - ÚPT 2019 RIV CZ eng C - Conference Paper (international conference)
Skoupý, Radim - Krzyžánek, Vladislav
Thickness determination of a cathodoluminescence active nanoparticles by means of Quantitative STEM imaging.
Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Proceedings of the 16th International Seminar. Brno: Institute of Scientific Instruments The Czech Academy of Sciences, 2018, s. 74-76. ISBN 978-80-87441-23-7.
[Recent Trends in Charged Particle Optics and Surface Physics Instrumentation. Skalský dvůr (CZ), 04.06.2018-08.06.2018]
R&D Projects: GA ČR GA17-15451S; GA MŠMT(CZ) LO1212; GA MŠMT ED0017/01/01
Institutional support: RVO:68081731
Keywords : quantitative imaging * Monte Carlo
OECD category: Nano-materials (production and properties)

Labeling of specimens by nanoscale probes is common approach of complex biological
systems exploration. Namely gold nanoparticles immuno-staining is well established method
in electron microscopy. However, if more than two label sizes are used, the differentiation of
individual nanoparticles becomes difficult.
This can be overcome by cathodoluminescence (CL) active particles – nanophosphors where
labels recognition is done by wavelength of emitted light. This gives a great opportunity to
use advanced multi probe labeling within one sample.
There is a huge variety of nanophosphors: green fluorescent protein, quantum dots, ZnO
nanoparticles, organic molecules, rare earth-doped nanophosphors etc. Therefore, in order
to choose best type of nanophosphors for a given task, it is important to measure particles
size/thickness, as the CL intensity is proportional to the probe volume.
Permanent Link: http://hdl.handle.net/11104/0287632