PLANT METABOLOMICS: METHODS AND PROTOCOLS

0494101 - ÚEB 2019 RIV US eng M - Monography Chapter
Novák, Ondřej - Floková, Kristýna
An UHPLC-MS/MS Method for Target Profiling of Stress-Related Phytohormones.
PLANT METABOLOMICS: METHODS AND PROTOCOLS. TOTOWA: HUMANA PRESS INC, 2018 - (Antonio, C.), s. 183-192. Methods in Molecular Biology. ISBN 978-1-4939-7819-9
R&D Projects: GA ČR(CZ) GA17-06613S
Institutional support: RVO:61389030
Keywords : Phytohormones * Jasmonates * Auxins
OECD category: Analytical chemistry

The methodology described here represents an improved strategy for analysis of a broad range of stress-related plant hormones including jasmonates, salicylic acid, abscisic acid, and auxin metabolites. The method conditions are optimized in order to reduce the background effect of complicated plant matrix, allow effective preconcentration and thus perform highly sensitive profiling of multiple plant hormones by ultrahigh performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).
Permanent Link: http://hdl.handle.net/11104/0287330