Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.]

0493934 - ÚEB 2019 RIV GB eng J - Journal Article
Gautier, F. - Eliášová, Kateřina - Leple, J.C. - Vondráková, Zuzana - Lomenech, A. M. - Le Metté, C. - Label, P. - Costa, G. - Trontin, J.F. - Teyssier, C. - Lelu-Walter, M.A.
Repetitive somatic embryogenesis induced cytological and proteomic changes in embryogenic lines of Pseudotsuga menziesii [Mirb.].
BMC Plant Biology. Roč. 18, AUG 10 (2018), č. článku 164. ISSN 1471-2229. E-ISSN 1471-2229
R&D Projects: GA MŠMT(CZ) LTC17030
Institutional support: RVO:61389030
Keywords : programmed cell-death * pinus-pinaster ait. * norway spruce * zygotic embryos * maritime pine * picea-abies * salicylic-acid * abiotic stress * abscisic-acid * larix * Douglas-fir * Embryogenic potential * Proliferation * Proteomic * Cytology * Plant growth regulators
OECD category: Plant sciences, botany
Impact factor: 3.670, year: 2018

To explore poorly understood differences between primary and subsequent somatic embryogenic lines of plants, we induced secondary (2ry) and tertiary (3ry) lines from cotyledonary somatic embryos (SEs) of two Douglas-fir genotypes: SD4 and TD17. The 2ry lines exhibited significantly higher embryogenic potential (SE yields) than the 1ry lines initiated from zygotic embryos (SD4, 2155 vs 477, TD17, 240 vs 29 g(-1) f.w.). Moreover, we observed similar differences in yield between 2ry and 3ry lines of SD4 (2400 vs 3921 g(-1) f.w.). To elucidate reasons for differences in embryogenic potential induced by repetitive somatic embryogenesis we then compared 2ry vs 1ry and 2ry vs 3ry lines at histo-cytological (using LC-MS/MS) and proteomic levels.
Permanent Link: http://hdl.handle.net/11104/0287197