Evaluation of extraction methods for quantitative analysis of tardigrade populations in soil and leaf litter

0493770 - FGÚ 2019 RIV DE eng J - Journal Article
Czerneková, Michaela - Jönsson, K. I. - Hajer, J. - Devetter, Miloslav
Evaluation of extraction methods for quantitative analysis of tardigrade populations in soil and leaf litter.
Pedobiologia. Roč. 70, Sep (2018), s. 1-5. ISSN 0031-4056. E-ISSN 1873-1511
R&D Projects: GA ČR(CZ) GAP207/12/0919
Institutional support: RVO:67985823 ; RVO:60077344
Keywords : tardigrades * water bears * extraction methods * soil * leaf litter
OECD category: Ecology; Zoology (BC-A)
Impact factor: 1.833, year: 2018

Accurate quantitative analysis of soil tardigrades depends on a relevant extraction method. Over the years, a number of different methods have been used, but the efficiency of these methods has rarely been evaluated for soil and leaf litter tardigrades. Four methods of extraction were compared in this study: the light-cooling (L-C) extractor, the high-gradient (H-G), Baermann extractor, the non-gradient (N-G) Baermann extractor and sieves. The results indicate that light and temperature are significant factors influencing tardigrade extraction. The L-C and H-G extractors were more effective than sieves for all substrate categories. These extraction methods (L-C and H-G) therefore seem to be appropriate for quantitative studies of soil and leaf litter tardigrades.
Permanent Link: http://hdl.handle.net/11104/0287081