What does it take to concentrate 20 ml sample volume by isotachophoresis?

0493601 - ÚIACH 2019 JP eng A - Abstract
Foret, František - Datinská, Vladimíra - Voráčová, Ivona - Gheibi, P. - Astier, Y. - Berka, J.
What does it take to concentrate 20 ml sample volume by isotachophoresis?
ITP2018. Final Program. Kyoto: Kyoto University at Katsura, 2018. KN-3.
[ITP 2018. International Symposium on Electro- and Liquid Phase-Separation Techniques /25./. 29.08.2018-01.09.2018, Kyoto]
R&D Projects: GA MŠMT(CZ) 8F17003
Institutional support: RVO:68081715
Keywords : isotachophoresis * sample concentration * DNA * capillary ITP
OECD category: Analytical chemistry

In conclusion, we have developed two simple, preparative methods for DNA concentration and purification. The first method, based on a capillary ITP, can process sample volumes up to 150 l. The second method, based on the flat channel design, allows processing of 15 ml sample volumes. This is by far the largest described focusing capacity.
Permanent Link: http://hdl.handle.net/11104/0286937