Separation of rotamers of 5-nitrosopyrimidines by capillary electrophoresis

0493511 - ÚOCHB 2019 CZ eng C - Conference Paper (international conference)
Štěpánová, Sille - Procházková, Eliška - Čechová, Lucie - Žurek, Jiří - Janeba, Zlatko - Dračínský, Martin - Kašička, Václav
Separation of rotamers of 5-nitrosopyrimidines by capillary electrophoresis.
Advances in Chromatography and Electrophoresis & Chiranal 2018. Olomouc: Palacký University, 2018 - (Bednář, P.; Cechová, M.; Kučera, L.; Kurka, O.), s. 130-131
[Advances in Chromatography and Electrophoresis & Chiranal 2018. Olomouc (CZ), 29.01.2018-01.02.2018]
R&D Projects: GA ČR(CZ) GA15-01948S; GA ČR(CZ) GA17-10832S
Institutional support: RVO:61388963
Keywords : rotamers * 5-nitrosopyrimidines * capillary electrophoresis
OECD category: Analytical chemistry

A new capillary electrophoresis (CE) method was developed for the separation of a new type of stereoisomers - rotamers of polysubstituted 5-nitrosopyrimidine derivatives. Partial separation of some rotamers was obtained in sodium borate, pH 9.3, or in Tris-phosphate, pH 2.2, background electrolytes (BGEs) free of cyclodextrins (CDs). However, the best, baseline separations of the rotamers of 5-nitrosopyrimidines were achieved in sodium borate BGE, pH 9.3, containing 20 mg/mL beta-CD as stereoselector, or in Tris-phosphate BGE, pH 2.2, with carboxymethyl-beta-CD as stereoselector.
Permanent Link: http://hdl.handle.net/11104/0286869