Affinity capillary electrophoresis applied to chiral separations of diquats and to determination of the stability constants of their complexes with sulfated cyclodextrins

0493495 - ÚOCHB 2019 CZ eng C - Conference Paper (international conference)
Kašička, Václav - Bílek, Jan - Koval, Dušan - Sázelová, Petra - Talele, Harish Ramesh - Severa, Lukáš - Reyes Gutierrez, Paul Eduardo - Teplý, Filip
Affinity capillary electrophoresis applied to chiral separations of diquats and to determination of the stability constants of their complexes with sulfated cyclodextrins.
Advances in Chromatography and Electrophoresis & Chiranal 2018. Olomouc: Palacký University, 2018 - (Bednář, P.; Cechová, M.; Kučera, L.; Kurka, O.), s. 56-57
[Advances in Chromatography and Electrophoresis & Chiranal 2018. Olomouc (CZ), 29.01.2018-01.02.2018]
R&D Projects: GA ČR(CZ) GA15-01948S; GA ČR(CZ) GA17-10832S
Institutional support: RVO:61388963
Keywords : affinity capillary electrophoresis * chiral separations * diquats
OECD category: Analytical chemistry

Affinity capillary electrophoresis (ACE) using an aqueous 22/35 mM sodium/phosphate buffer, pH 2.5, as the background electrolyte (BGE), and 6 mM randomly highly sulfated alpha-, beta- and gamma-cyclodextrins (CDs) as chiral selectors has been successfully applied for separation of (P)- and (M)-enantiomers of a series of eleven new diquats (DQs) and for estimation of the strength of their complexes with the above CDs. The apparent stability constants of the DQs-CDs complexes were determined from the simultaneous ACE measurements of the dependence of effective electrophoretic mobilities of (P)- and (M)-enantiomers of DQs on the concentrations of CDs in the BGE by nonlinear regression analysis. The DQs enantiomers formed strong complexes with all three types of CDs with the apparent stability constants in the range (7.80 547.4) × 103 L/mol.
Permanent Link: http://hdl.handle.net/11104/0286857