sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements

0493283 - MBÚ 2019 RIV US eng J - Journal Article
Ramaniuk, Olga - Převorovský, M. - Pospíšil, Jiří - Vítovská, Dragana - Kofroňová, Olga - Benada, Oldřich - Schwarz, Marek - Šanderová, Hana - Hnilicová, Jarmila - Krásný, Libor
sigma(I) from Bacillus subtilis: Impact on Gene Expression and Characterization of sigma(I)-Dependent Transcription That Requires New Types of Promoters with Extended-35 and-10 Elements.
Journal of Bacteriology. Roč. 200, č. 17 (2018), č. článku e00251-18. ISSN 0021-9193. E-ISSN 1098-5530
R&D Projects: GA MZd(CZ) NV17-29680A; GA MŠMT(CZ) LM2015055; GA MŠMT(CZ) LO1509
Institutional support: RVO:61388971
Keywords : RNAP * RNA-seq * iron metabolism
OECD category: Microbiology
Impact factor: 3.234, year: 2018

The sigma(I) sigma factor from Bacillus subtilis is a a factor associated with RNA polymerase (RNAP) that was previously implicated in adaptation of the cell to elevated temperature. Here, we provide a comprehensive characterization of this transcriptional regulator. By transcriptome sequencing (RNA-seq) of wild-type (wt) and sigma(I)-null strains at 37 degrees C and 52 degrees C, we identified -130 genes affected by the absence of sigma(I). Further analysis revealed that the majority of these genes were affected indirectly by sigma(I). The sigma(I) regulon, i.e., the genes directly regulated by sigma(I), consists of 16 genes, of which eight (the dhb and yku operons) are involved in iron metabolism. The involvement of sigma(I) in iron metabolism was confirmed phenotypically. Next, we set up an in vitro transcription system and defined and experimentally validated the promoter sequence logo that, in addition to -35 and -10 regions, also contains extended -35 and -10 motifs. Thus, sigma(I)-dependent promoters are relatively information rich in comparison with most other promoters. In summary, this study supplies information about the least-explored sigma factor from the industrially important model organism B. subtilis.

Permanent Link: http://hdl.handle.net/11104/0286673