G protein-coupled estrogen receptor (GPER) in adult boar testes, epididymis and spermatozoa during epididymal maturation

0492930 - BTÚ 2019 RIV GB eng J - Journal Article
Krejčířová, R. - Manasova, M. - Sommerová, V. - Langhamerova, E. - Rajmon, R. - Maňásková-Postlerová, Pavla
G protein-coupled estrogen receptor (GPER) in adult boar testes, epididymis and spermatozoa during epididymal maturation.
International Journal of Biological Macromolecules. Roč. 116, SEP 2018 (2018), s. 113-119. ISSN 0141-8130. E-ISSN 1879-0003
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109
Institutional support: RVO:86652036
Keywords : GPER * Adult boar testes * Epididymal spermatozoa
OECD category: Biochemistry and molecular biology
Impact factor: 4.784, year: 2018

The G protein-coupled estrogen receptor (GPER) is a transmembrane receptor considered as a mediator of rapid non-genomic responses. GPER has been found in the male reproductive tract of many mammalian species. However, in adult boars, GPER has been reported only in ejaculated spermatozoa. Therefore, we focused on GPER detection in testicular and epididymal tissues and sperm cells in adult boars. We found GPER in Leydig cells and seminiferous tubules of boar testes and in the secretory epithelium of epididymis. A weaker signal was visible in smooth muscle cells and spermatozoa in the epididymal tubule. In spermatozoa isolated from epididymal parts, GPER was found to localize mainly in the sperm acrosome and flagellum. We immunodetected several protein bands in the extracts of the tissues and epididymal spermatozoa. A significantly higher amount of GPER mRNA was detected in the spermatozoa from caput epididymis, whereas the mRNA expression was lower in tissues of testes and caput epididymal. Our results showed the first evidence of GPER in boar epididymal spermatozoa. Moreover, the GPER localization in adult boar testes, epididymis, and mature spermatozoa suggests the involvement of estrogens via transmembrane receptor and rapid non-genomic signaling in both the sperm development and post-testicular maturation. (C) 2018 Elsevier B.V. All rights reserved.
Permanent Link: http://hdl.handle.net/11104/0286400