Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization

0492597 - MBÚ 2019 RIV US eng J - Journal Article
Svobodová, Kateřina - Semerád, Jaroslav - Petráčková, Denisa - Novotný, Čeněk
Antibiotic Resistance in Czech Urban Wastewater Treatment Plants: Microbial and Molecular Genetic Characterization.
Microbial Drug Resistance-Mechanisms Epidemiology and Disease. Roč. 24, č. 6 (2018), s. 830-838. ISSN 1076-6294. E-ISSN 1931-8448
Institutional support: RVO:61388971
Keywords : wastewater treatment plants * antibiotic resistance genes * resistome
OECD category: Microbiology
Impact factor: 2.397, year: 2018

Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, bla(TEM), ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100g/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.
Permanent Link: http://hdl.handle.net/11104/0286062