A Combinatorial Lipid Code Shapes the Electrostatic Landscape of Plant Endomembranes

0492203 - ÚEB 2019 RIV US eng J - Journal Article
Platre, M.P. - Noack, H. - Doumane, M. - Bayle, P. - Simon, M.L.A. - Maneta-Peyret, L. - Fouillen, L. - Stanislas, T. - Armengot, L. - Pejchar, Přemysl - Caillaud, T. - Potocký, Martin - Čopič, A. - Moreau, P. - Jaillais, Y.
A Combinatorial Lipid Code Shapes the Electrostatic Landscape of Plant Endomembranes.
Developmental Cell. Roč. 45, č. 4 (2018), s. 465-480. ISSN 1534-5807. E-ISSN 1878-1551
R&D Projects: GA ČR GA17-27477S
Institutional support: RVO:61389030
Keywords : arabidopsis-thaliana * phosphatidic-acid * plasma-membrane * receptor activation * image-analysis * cells * trafficking * transport * compartments * curvature
OECD category: Cell biology
Impact factor: 9.190, year: 2018

Membrane surface charge is critical for the transient, yet specific recruitment of proteins with polybasic regions to certain organelles. In eukaryotes, the plasma membrane (PM) is the most electronegative compartment of the cell, which specifies its identity. As such, membrane electrostatics is a central parameter in signaling, intracellular trafficking, and polarity. Here, we explore which are the lipids that control membrane electrostatics using plants as a model. We show that phosphatidylinositol-4-phosphate (PI4P), phosphatidic acidic (PA), and phosphatidylserine (PS) are separately required to generate the electrostatic signature of the plant PM. In addition, we reveal the existence of an electrostatic territory that is organized as a gradient along the endocytic pathway and is controlled by PS/PI4P combination. Altogether, we propose that combinatorial lipid composition of the cytosolic leaflet of organelles not only defines the electrostatic territory but also distinguishes different functional compartments within this territory by specifying their varying surface charges.
Permanent Link: http://hdl.handle.net/11104/0285751