Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics

0492200 - ÚEB 2019 RIV US eng J - Journal Article
Šimura, Jan - Antoniadi, I. - Široká, Jitka - Tarkowská, Danuše - Strnad, Miroslav - Ljung, K. - Novák, Ondřej
Plant Hormonomics: Multiple Phytohormone Profiling by Targeted Metabolomics.
Plant Physiology. Roč. 177, č. 2 (2018), s. 476-489. ISSN 0032-0889. E-ISSN 1532-2548
R&D Projects: GA MŠMT(CZ) LO1204; GA ČR(CZ) GA17-06613S
Institutional support: RVO:61389030
Keywords : tandem mass-spectrometry * solid-phase extraction * arabidopsis root apex * liquid-chromatography * abscisic-acid * major phytohormones * sample preparation * isotope-dilution * uhplc-ms/ms * cytokinins
OECD category: Plant sciences, botany
Impact factor: 6.305, year: 2018

Phytohormones are physiologically important small molecules that play essential roles in intricate signaling networks that regulate diverse processes in plants. We present a method for the simultaneous targeted profiling of 101 phytohormone-related analytes from minute amounts of fresh plant material (less than 20 mg). Rapid and nonselective extraction, fast one-step sample purification, and extremely sensitive ultra-high-performance liquid chromatography-tandem mass spectrometry enable concurrent quantification of the main phytohormone classes: cytokinins, auxins, brassinosteroids, gibberellins, jasmonates, salicylates, and abscisates. We validated this hormonomic approach in salt-stressed and control Arabidopsis (Arabidopsis thaliana) seedlings, quantifying a total of 43 endogenous compounds in both root and shoot samples. Subsequent multivariate statistical data processing and cross-validation with transcriptomic data highlighted the main hormone metabolites involved in plant adaptation to salt stress.
Permanent Link: http://hdl.handle.net/11104/0285748