Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis

0492193 - ÚIACH 2019 RIV CZ eng C - Conference Paper (international conference)
Vaněčková, T. - Zítka, J. - Hlaváček, Antonín - Adam, V. - Vaculovičová, M.
Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis.
MendelNet 2017: Proceedings of International PhD Students Conference. Brno: Mendelova univerzita, 2017 - (Cerkal, R.; Březinová Belceredi, N.; Prokešová, L.; Vacek, P.), s. 953-957. ISBN 978-80-7509-529-9.
[MendelNet 2017: International PhD Students Conference /24./. Brno (CZ), 08.11.2017-09.11.2017]
R&D Projects: GA ČR(CZ) GBP206/12/G014
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * fluorescence spectrometry * upconversion nanoparticles
OECD category: Physical chemistry
https://mnet.mendelu.cz/mendelnet2017/mnet_2017_full.pdf

Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.
Permanent Link: http://hdl.handle.net/11104/0285736