Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica

0492151 - BÚ 2019 RIV DE eng J - Journal Article
Pushkareva, E. - Pessi, I. S. - Namsaraev, Z. - Mano, M. J. - Elster, Josef - Wilmotte, A.
Cyanobacteria inhabiting biological soil crusts of a polar desert: Sør Rondane Mountains, Antarctica.
Systematic and Applied Microbiology. Roč. 41, č. 4 (2018), s. 363-373. ISSN 0723-2020. E-ISSN 1618-0984
Institutional support: RVO:67985939
Keywords : Cyanobacteria * Biological Soil Crusts * Antarctica
OECD category: Ecology
Impact factor: 2.808, year: 2018

Molecular and morphological methods were applied to study cyanobacterial community composition in biological soil crusts (BSCs) from four areas (two nunataks and two ridges) in the Sør Rondane Mountains, Antarctica. The sampling sites serve as control areas for open top chambers (OTCs) which were placed in 2010 at the time of sample collection and will be compared with BSC samples taken from the OTCs in future. Cyanobacterial cell biovolume was estimated using epifluorescence microscopy, which revealed the dominance of filamentous cyanobacteria in all studied sites except the Utsteinen ridge, where unicellular cyanobacteria were the most abundant. Cyanobacterial diversity was studied by a combination of molecular fingerprinting methods based on the 16S rRNA gene (denaturing gradient gel electrophoresis (DGGE) and 454 pyrosequencing) using cyanobacteria specific primers.
Permanent Link: http://hdl.handle.net/11104/0285711