Study of various parameters that influence the content of exhaled breath condensate used in the diagnosis of gastroesophageal reflux disease

0492061 - ÚIACH 2019 RIV HU eng J - Journal Article
Greguš, M. - Ďurč, P. - Lačná, J. - Foret, František - Kubáň, Petr
Study of various parameters that influence the content of exhaled breath condensate used in the diagnosis of gastroesophageal reflux disease.
Hungarian Journal of Industrial Chemistry. Roč. 46, č. 1 (2018), s. 29-34. ISSN 0133-0276
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * exhaled breath condensate * ionic analysis
OECD category: Analytical chemistry

In this work, various parameters that influence the ionic content and pH of exhaled breath condensate in terms of the non-invasive diagnosis of gastroesophageal reflux disease were studied. Exhaled breath condensate samples were collected using a miniature and inexpensive sampling device. Capillary electrophoresis with contactless conductometric detection was used to monitor the ionic content of exhaled breath condensate. Background electrolyte composed of 20 mM of 2-(N-Morpholino)ethanesulfonic acid, 20 mM of L-Histidine, 2 mM of 18-Crown-6 and 30 mu M of cetyltrimethylammonium bromide facilitated the rapid separation of anions and cations, both in less than 2 minutes. The possibility of contamination of the exhaled breath condensate by saliva is discussed in detail. The day-to-day repeatability (n=5) of the ionic content and pH of the exhaled breath condensate was studied and was satisfactory, reflecting mainly the physiological variability.
Permanent Link: http://hdl.handle.net/11104/0285633