Nanomaterials in Biomedical research

0491806 - ÚEM 2019 RIV CZ eng M - Monography Chapter
Hájková, Michaela - Heřmánková, Barbora - Krulová, Magdaléna
Study of Synergistic Effect of Mesenchymal Stem Cells and Immunosuppressive Drugs on an Inflammatory Response in an In Vitro Model.
Nanomaterials in Biomedical research. Praha 3: Proxima Tisk, s.r.o., 2017 - (Caraglia, M.), s. 124-128. ISBN 978-80-906655-5-2
R&D Projects: GA MŠMT(CZ) LO1309
Institutional support: RVO:68378041
Keywords : mesenchymal stem cells * cyclosporine A * mycophenolate mofetil
OECD category: Cell biology

Mesenchymal stem cells (MSCs) in combination with immunosuppressive drugs are beneficial in the treatment of inflammatory diseases or transplantation reaction. We tested immunosuppressive drugs with different mechanism of action: cyclosporine A, mycophenolate mofetil and rapamycin in combination with MSCs. By flow cytometry we determined T regulatory cells (CD4+Foxp3+) and Th 17 cells (CD4+RORt+) proportion and the production of IL-17 was measured by ELISA. This study demonstrated that MSCs in combination with different immunosuppressive agents at low concentration have distinct effect on the Treg/Th17 balance.
Permanent Link: http://hdl.handle.net/11104/0291418