Fluorescence & bioluminescence in the quest for imaging, probing & analysis of mycobacterial infections

0491291 - ÚMCH 2019 RIV GB eng J - Journal Article
Trousil, Jiří - Ulmann, V. - Hrubý, Martin
Fluorescence & bioluminescence in the quest for imaging, probing & analysis of mycobacterial infections.
Future Microbiology. Roč. 13, č. 8 (2018), s. 933-951. ISSN 1746-0913. E-ISSN 1746-0921
R&D Projects: GA ČR(CZ) GA17-07164S; GA ČR(CZ) GA17-09998S
Institutional support: RVO:61389013
Keywords : fluorescence and bioluminescence * mycobacteria * tuberculosis
OECD category: Polymer science
Impact factor: 2.746, year: 2018

Mycobacterioses represent a global health problem and rapid diagnostic improvements are urgently required. Mycobacteria-specific fluorescence and bioluminescence phenomena have been found to be useful for a wide range of mycobacteria-focused research. Here, we present a critical survey of the most promising techniques in this field and the potential of new methods under investigation. These approaches include acid-fast staining, intrinsic fluorescence of the coenzyme F420, fluorogenic substrates (e.g., β-lactamase-sensitive coumpounds) and recombination of mycobacteria or mycobacteriophages. Probably the most interesting and emerging host-inspecting approach is in vivo imaging. Detection of fluorescence in vivo, however, is complicated by light scattering, light absorption, and autofluorescence, caused by the tissues. Despite this, many of these systems show promise as the foundations for improved rapid analysis and imaging of mycobacterial infections, both in vitro and in vivo.
Permanent Link: http://hdl.handle.net/11104/0285389