Selected human sperm proteins as possible immunogens contributing to idiopathic infertility in woman.\n

0491142 - BTÚ 2019 CZ eng A - Abstract
Páleníková, Veronika - Krátká, Z. - Luxová, S. - Malíčková, K. - Dvořáková-Hortová, Kateřina - Postlerová, Pavla
Selected human sperm proteins as possible immunogens contributing to idiopathic infertility in woman.
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Abstract book of XXIVth Symposium of Immunology and Biology of Reproduction. Vestec: Biotechnologický ústav AVČR v. v. i., 2018 - (Pěknicová, J.). s. 29-29
[XXIVth Symposium of Immunology and Biology of Reproduction. 17.05.2018-19.05.2018, Třešť]
R&D Projects: GA MŠMT(CZ) ED1.1.00/02.0109; GA ČR(CZ) GA18-11275S
Institutional support: RVO:86652036
Keywords : human sperm proteins * infertility * ASA-antigens
OECD category: Biochemistry and molecular biology

Immunological infertility is a problem for many couples. The immune system may jeopardize fertilization in many aspects, including production of anti-sperm antibodies (ASA). These antibodies bind to the different antigens of spermatozoa and affect the sperm motility, sperm-egg interaction or fusion. The large number of ASA-antigens decreases the quality of diagnostic methods and consequently the treatment. Therefore, our aim was to identify immunogens in ejaculate samples based on their response to the serum of women suffering with idiopathic infertility. The goal was to characterize several common ASA antigens that could lead to a better understanding of principles and mechanisms of these antibodies in order to open new diagnostic and therapeutic possibilities. Methodologically, we isolated proteins from seminal plasma and spermatozoa from ejaculates of patients with different spermiograms. These proteins were separated by SDS-electrophoresis and subsequently subjected to the Western blot analysis. Membranes were incubated with sera of women and detected via secondary antibody against different classes of human immunoglobulins. The obtained results were compared with data from spermiograms, immunoglobulins and women sera. Every sperm samples contained great amount antibody-reactive proteins, and we found several repeated antigens with molecular masses of approximately 75 kDa, 37 kDa and 15 kDa. In addition, serum of an each patient contained ASA that gave unique response to an each male sample. Individually based interaction between women sera and sperm samples is reason why it is so important to establish a common ASA-binding protein for efficient diagnostic tests and subsequently improve therapeutic approaches.
Permanent Link: http://hdl.handle.net/11104/0286610