Electrophoretic focusing on inverse electromigration dispersion gradient. The fundamental resolution equation and pressure-assisted performance enhancement

0490545 - ÚIACH 2019 RIV NL eng J - Journal Article
Malá, Zdeňka - Gebauer, Petr
Electrophoretic focusing on inverse electromigration dispersion gradient. The fundamental resolution equation and pressure-assisted performance enhancement.
Journal of Chromatography A. Roč. 1563, AUG (2018), s. 191-197. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA16-09135S
Institutional support: RVO:68081715
Keywords : capillary electrophoresis * focusing * sulfonamides * chlorophenols
OECD category: Analytical chemistry
Impact factor: 3.858, year: 2018

Electrophoretic focusing on inverse electromigration dispersion (EMD) gradient is a new analytical technique based on a unique separation principle where weak non-amphoteric ionogenic species are focused, separated and transported to the detector by an EMD profile of suitable properties. The present work extends the theoretical description of this method by introducing the concept of resolution and deriving the fundamental equation expressing resolution as function of basic system parameters. The results indicate that at constant current operation, resolution is proportional to the square root of time. For variable current regimes (e.g. constant voltage), the time variable is replaced by the product of electric current and passed electric charge. Computer simulations for a model pair of substances support the validity of the presented theory and confirm the theoretical conclusion that resolution can be increased by allowing longer electromigration of the gradient in terms of time or passed charge.
Permanent Link: http://hdl.handle.net/11104/0284734