Enantiomeric separation of triacylglycerols containing very long chain fatty acids

0490505 - MBÚ 2019 RIV NL eng J - Journal Article
Řezanka, Tomáš - Kolouchová, I. - Nedbalová, L. - Sigler, Karel
Enantiomeric separation of triacylglycerols containing very long chain fatty acids.
Journal of Chromatography A. Roč. 1557, JUL 6 (2018), s. 9-19. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA17-00027S
Institutional support: RVO:61388971
Keywords : Triacylglycerols * Very long chain fatty acids * Very long-chain polyunsaturated fatty acids
OECD category: Microbiology
Impact factor: 3.858, year: 2018

Enantiomers of triacylglycerols (TAGs) containing any combination of very long chain fatty acids (VLCFAs) and/or very long chain polyunsaturated fatty acids (VLCPUFAs) with diolein, dilinolein and didocosahexaenoin were synthesized. Gradient non-aqueous reversed-phase high-performance liquid chromatography/high resolution atmospheric pressure chemical ionization-tandem mass spectrometry (NARP-HPLC/HRMS2-APCI) and chiral liquid chromatography were used for the separation and identification of molecular species of these TAGs. Further, NARP-LC and chiral LC were used to separate natural mixtures of TAGs obtained from four natural sources, i.e. ximenia oil (Ximenia americana), green alga (Botryococcus braunii), breweris yeast (Saccharomyces pastorianus) and a dinoflagellate (Amphidinium carterae). The ratio of regioisomers and enantiomers in individual samples was determined and a hypothesis has been confirmed on the biosynthetic pathway of natural TAGs, which is based on the preferential representation of VLCFAs and VLCPUFAs in the sn-1 position of the glycerol backbone.
Permanent Link: http://hdl.handle.net/11104/0284727