Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography

0490130 - ÚIACH 2019 RIV NL eng J - Journal Article
Moravcová, Dana - Planeta, Josef - King, A. W. T. - Wiedmer, S. K.
Immobilization of a phosphonium ionic liquid on a silica monolith forhydrophilic interaction chromatography.
Journal of Chromatography A. Roč. 1552, JUN (2018), s. 53-59. ISSN 0021-9673. E-ISSN 1873-3778
R&D Projects: GA ČR(CZ) GA16-03749S; GA MV VI20172020069
Institutional support: RVO:68081715
Keywords : silica monolithic capillary column * HILIC * nucleobase * nucleoside
OECD category: Analytical chemistry
Impact factor: 3.858, year: 2018

A methodology for preparing phosphonium-based ionic liquid modified silica-based monolithic cap-illary columns is presented. The silica monolithic columns with dimensions of 150 × 0.1 mm weremodified by a phosphonium-based ionic liquid (trioctyl(3/4-vinylbenzyl)phosphonium chloride) via3-(trimethoxysilyl)propyl methacrylate. The prepared columns were evaluated under hydrophilic inter-action liquid chromatography separation conditions, employing a sample mixture containing purine andpyrimidine bases and nucleosides. Detection was made by UV. The high efficiency of the original silicamonolith was preserved even after modification, and it reached values in the range of 98,000–174,000theoretical plates/m. The effects of the concentration of acetonitrile in the mobile phase, the presence ofadditives in the mobile phase, such as, acetic acid or ammonium acetate, and the pH of the mobile phaseon the separation of some selected analytes were investigated. The prepared columns showed differentseparation selectivity compared to silica, phenyl and sulfobetaine stationary phases.
Permanent Link: http://hdl.handle.net/11104/0284419