HPLC-ED/UV with Solid Phase Extraction for the Determination of 5-Hydroxyindole-3-acetic Acid

0490024 - ÚFCH JH 2020 RIV CZ eng C - Conference Paper (international conference)
Němečková-Makrlíková, Anna - Dejmková, H. - Navrátil, Tomáš - Barek, J. - Vyskočil, V.
HPLC-ED/UV with Solid Phase Extraction for the Determination of 5-Hydroxyindole-3-acetic Acid.
XXXVIII. Moderní elektrochemické metody. Sborník přednášek. Ústí nad Labem: Best Servis, 2018 - (Navrátil, T.; Fojta, M.; Schwarzová, K.), s. 147-150. ISBN 978-80-905221-6-9.
[Moderní elektrochemické metody /38./. Jetřichovice (CZ), 21.05.2018-25.05.2018]
R&D Projects: GA ČR GA17-03868S
Institutional support: RVO:61388955
Keywords : HPLC * electrochemical detection * spectrophotometric detection
OECD category: Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Determination of 5-HIAA in human urine was successfully performed using HPLC-ED/UV with SPE. HPLC-ED is a very sensitive technique, presented method does not need any sample pre-treatment apart from SPE procedure, which replaced filtration of samples of human urine to avoid problems with clogging a HPLC column. The found native
concentrations of 5-HIAA in human urine using HPLC-ED with SPE fully corresponds with published normal concentrations in urine (17.8 - 58.3 micromol l-1). The found native concentrations of 5-HIAA were 18.4 micromol L-1 (calculated from peak height). The presented method is fast and could be used for simple
screening of human urine, because HPLC-ED/UV allows simultaneous determination of 5-HIAA, HVA, and VMA as important tumour biomarkers.
Permanent Link: http://hdl.handle.net/11104/0284335