Number of the records: 1  

A human pericardium biopolymeric scaffold for autologous heart valve tissue engineering: cellular and extracellular matrix structure and biomechanical properties in comparison with a normal aortic heart valve

  1. 1.
    0489876 - FGÚ 2019 RIV NL eng J - Journal Article
    Straka, František - Schorník, David - Mašín, J. - Filová, Elena - Miřejovský, T. - Burdíková, Z. - Švindrych, Z. - Chlup, H. - Horný, L. - Daniel, M. - Machač, Jiří - Skibová, J. - Pirk, J. - Bačáková, Lucie
    A human pericardium biopolymeric scaffold for autologous heart valve tissue engineering: cellular and extracellular matrix structure and biomechanical properties in comparison with a normal aortic heart valve.
    Journal of Biomaterial Science Polymer Edition. Roč. 29, č. 6 (2018), s. 599-634. ISSN 0920-5063. E-ISSN 1568-5624
    R&D Projects: GA MZd(CZ) NT11270; GA MZd(CZ) NV15-29153A
    Institutional support: RVO:67985823 ; RVO:67985939
    Keywords : autologous * human pericardium * pericardial interstitial cells * human aortic heart valve * valvular interstitial cells * extracellular matrix * collagen * elastin * glycosaminoglycans * secant elastic modulus
    OECD category: Cardiac and Cardiovascular systems
    Impact factor: 2.121, year: 2018

    The objective of our study was to compare the cellular and extracellular matrix (ECM) structure and the biomechanical properties of human pericardium (HP) with the normal human aortic heart valve (NAV). HP tissues (from 12 patients) and NAV samples (from 5 patients) were harvested during heart surgery. The main cells in HP were pericardial interstitial cells, which are fibroblast-like cells of mesenchymal origin similar to the valvular interstitial cells in NAV tissue. The ECM of HP had a statistically significantly (p< 0.001) higher collagen I content, a lower collagen III and elastin content, and a similar glycosaminoglycans (GAGs) content, in comparison with the NAV, as measured by ECM integrated density. However, the relative thickness of the main load-bearing structures of the two tissues, the dense part of fibrous HP (49 +/- 2%) and the lamina fibrosa of NAV (47 +/- 4%), was similar. In both tissues, the secant elastic modulus (Es) was significantly lower in the transversal direction (p< 0.05) than in the longitudinal direction. This proved that both tissues were anisotropic. No statistically significant differences in UTS (ultimate tensile strength) values and in calculated bending stiffness values in the longitudinal or transversal direction were found between HP and NAV. Our study confirms that HP has an advantageous ECM biopolymeric structure and has the biomechanical properties required for a tissue from which an autologous heart valve replacement may be constructed.
    Permanent Link: http://hdl.handle.net/11104/0284291

     
     
Number of the records: 1  

  This site uses cookies to make them easier to browse. Learn more about how we use cookies.