Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains

0489867 - FGÚ 2019 RIV CZ eng J - Journal Article
Kolínská, Jiřina - Zákostelecká, Marie - Zemanová, Zdeňka - Lisá, Věra - Goliáš, Jaroslav - Kozáková, Hana - Dvořák, B.
Cellular differentiation of non-transformed intestinal epithelial cells is regulated by Lactobacillus rhamnosus and L. casei strains.
Physiological Research. Roč. 67, č. 2 (2018), s. 261-273. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MŠMT(CZ) ME10017; GA MŠMT(CZ) 7AMB16PL006
Institutional support: RVO:67985823 ; RVO:61388971
Keywords : IEC-18 * Lactobacillus * IL-18 * TLRs * Caspase-1 * Villin * beta-catenin
OECD category: Physiology (including cytology)
Impact factor: 1.701, year: 2018

The aim of this study was to characterize an in vitro modulating effect of three commensal Lactobacillus strains on cellular differentiation of non-transformed crypt-like rat small intestinal cell line IEC-18. IEC-18 was grown on extracellular matrix, with or without presence of Lactobacillus strains. Gene expression of IEC-18 bacterial detection system - such as Toll-like receptors TLR-2, TLR-4, signal adapter MyD88, cytoplasmic NOD2 receptor, inflammatory cytokines IL-18, IL-1 beta, chemokine IL-8 and enzyme caspase-1 - was evaluated using real-time PCR. Expression and localization of TLR-2, TLR-4, IL-18 and caspase-1 proteins was demonstrated by Western blotting and immunofluorescent staining. Secretion of IL-18 to apical and basolateral surfaces was assayed by ELISA. Our results suggested that L. casei LOCK0919 accelerated differentiation of IEC-18 by stimulating TLR-2, TLR-4, MyD88, IL-18, caspase-1 mRNAs and proteins. L. casei LOCK0919 increased expression and transfer of villin and beta-catenin from cytoplasm to cell membrane. Presence of L. rhamnosus LOCK0900 resulted in detachment of IEC-18 layer from extracellular matrix leading to induction of IL-1 beta, of TLR-2 and IL-8 mRNAs and stimulation of MyD88, caspase-1 and cytosolic receptor NOD2 mRNAs. L. rhamnosus LOCK0908 was not recognized by TLR-2 or TLR-4 receptors. Lactobacilli-IEC-18 crosstalk enhanced immune and barrier mucosal functions.
Permanent Link: http://hdl.handle.net/11104/0284185