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High-contrast differentiation resolution 3D imaging of rodent brain by X-ray computed

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    0489562 - ÚŽFG 2019 RIV GB eng J - Journal Article
    Zikmund, T. - Novotná, M. - Kavková, M. - Tesařová, M. - Kaucká, M. - Szarowská, B. - Idameyko, I. - Hrubá, Eva - Buchtová, Marcela - Dražanová, Eva - Starčuk, Zenon - Kaiser, J.
    High-contrast differentiation resolution 3D imaging of rodent brain by X-ray computed.
    Journal of Instrumentation. Roč. 13, č. 1 (2018), č. článku C02039. ISSN 1748-0221. E-ISSN 1748-0221
    R&D Projects: GA MŠMT EF15_003/0000460; GA ČR(CZ) GB14-37368G
    Institutional support: RVO:67985904 ; RVO:68081731
    Keywords : computerized tomography * computed radiography * MRI
    OECD category: Technologies involving the manipulation of cells, tissues, organs or the whole organism (assisted reproduction); Medical engineering (UPT-D)
    Impact factor: 1.366, year: 2018

    The biomedically focused brain research is largely performed on laboratory mice considering a high homology between the human and mouse genomes. A brain has an intricate and highly complex geometrical structure that is hard to display and analyse using only 2D methods. Applying some fast and efficient methods of brain visualization in 3D will be crucial for the neurobiology in the future. A post-mortem analysis of experimental animals' brains usually involves techniques such as magnetic resonance and computed tomography. These techniques are employed to visualize abnormalities in the brains' morphology or reparation processes. The X-ray computed microtomography (micro CT) plays an important role in the 3D imaging of internal structures of a large variety of soft and hard tissues. This non-destructive technique is applied in biological studies because the lab-based CT devices enable to obtain a several-micrometer resolution. However, this technique is always used along with some visualization methods, which are based on the tissue staining and thus differentiate soft tissues in biological samples. Here, a modified chemical contrasting protocol of tissues for a micro CT usage is introduced as the best tool for ex vivo 3D imaging of a post-mortem mouse brain. This way, the micro CT provides a high spatial resolution of the brain microscopic anatomy together with a high tissue differentiation contrast enabling to identify more anatomical details in the brain. As the micro CT allows a consequent reconstruction of the brain structures into a coherent 3D model, some small morphological changes can be given into context of their mutual spatial relationships.
    Permanent Link: http://hdl.handle.net/11104/0283958

     
     
Number of the records: 1  

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