Immobilization in polyvinyl alcohol hydrogel enhances yeast storage stability and reusability of recombinant laccase-producing S-cerevisiae

0489311 - FGÚ 2019 RIV NL eng J - Journal Article
Herkommerová, Klára - Zemančíková, Jana - Sychrová, Hana - Antošová, Zuzana
Immobilization in polyvinyl alcohol hydrogel enhances yeast storage stability and reusability of recombinant laccase-producing S-cerevisiae.
Biotechnology Letters. Roč. 40, č. 2 (2018), s. 405-411. ISSN 0141-5492. E-ISSN 1573-6776
R&D Projects: GA TA ČR(CZ) TA01011461
Institutional support: RVO:67985823
Keywords : immobilization * laccase * LentiKats * polyvinyl alcohol hydrogel * reusability * storage stability * yeasts
OECD category: Industrial biotechnology
Impact factor: 2.154, year: 2018

To improve the storage stability and reusability of various yeast strains and species by immobilization in polyvinyl alcohol (PVA) hydrogel particles.Debaryomyces hansenii, Pichia sorbitophila, Saccharomyces cerevisiae, Yarrowia lipolytica, and Zygosaccharomyces rouxii were immobilized in PVA particles using LentiKats technology and stored in sterile water at 4 A degrees C. The immobilization improved the survival of all species, however, the highest storage stability was achieved for S. cerevisiae and Y. lipolytica which survived more than 1 year, in contrast to free cells that survived for only 3 months. Tests of the reusability of immobilized recombinant laccase-secreting S. cerevisiae revealed that the cells were suitable for repetitive use (55 cycles during 15 months) even after storage in water at 4 A degrees C for 9 months. A suitable method for killing immobilized laccase-secreting cells without affecting the produced enzyme activity was also developed. The immobilization of yeasts in PVA hydrogel enables long-term, cheap storage with very good cell viability and productivity, thus becoming a promising approach for industrial applications.
Permanent Link: http://hdl.handle.net/11104/0283752