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Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters
- 1.0489305 - FGÚ 2019 RIV NL eng J - Journal Article
Zemančíková, Jana - Dušková, Michala - Elicharová, Hana - Papoušková, Klára - Sychrová, Hana
Osmotolerance of Dekkera bruxellensis and the role of two Stl glycerol–proton symporters.
FEMS Microbiology Letters. Roč. 365, č. 5 (2018), č. článku fny020. ISSN 0378-1097. E-ISSN 1574-6968
R&D Projects: GA ČR(CZ) GA15-03708S
Institutional support: RVO:67985823
Keywords : glycerol uptake * osmotolerance * non-conventional yeasts
OECD category: Microbiology
Impact factor: 1.994, year: 2018
Dekkera bruxellensis is important for lambic beer fermentation but is considered a spoilage yeast in wine fermentation. We compared two D. bruxellensis strains isolated from wine and found that they differ in some basic properties, including osmotolerance. The genomes of both strains contain two highly similar copies of genes encoding putative glycerol–proton symporters from the STL family that are important for yeast osmotolerance. Cloning of the two DbSTL genes and their expression in suitable osmosensitive Saccharomyces cerevisiae mutants revealed that both identified genes encode functional glycerol uptake systems, but only DbStl2 has the capacity to improve the osmotolerance of S. cerevisiae cells.
Permanent Link: http://hdl.handle.net/11104/0283748
Number of the records: 1