Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome

0489197 - FGÚ 2019 RIV US eng J - Journal Article
Mikšík, Ivan - Pataridis, Statis - Eckhardt, Adam - Sedmera, David
Analysis of Siamese Crocodile (Crocodylus siamensis) Eggshell Proteome.
Protein Journal. Roč. 37, č. 1 (2018), s. 21-37. ISSN 1572-3887. E-ISSN 1573-4943
R&D Projects: GA ČR(CZ) GA15-01948S; GA ČR(CZ) GA16-02972S
Institutional support: RVO:67985823
Keywords : proteins of eggshell * eggshell pigment * crocodile
OECD category: Analytical chemistry
Impact factor: 1.029, year: 2018

The proteins and pigment of the eggshell of the Siamese crocodile (Crocodylus siamensis) were analysed. For proteomic analysis, various decalcification methods were used when the two main surface layers were analyzed. These layers are important for antimicrobial defense of egg (particularly the cuticle). We found 58 proteins in both layers, of which 4 were specific for the cuticle and 26 for the palisade (honeycomb) layer. Substantial differences between proteins in the eggshell of crocodile and previously described birds' eggshells exist (both in terms of quality and quantity), however, the entire proteome of Crocodilians has not been described yet. The most abundant protein was thyroglobulin. The role of determined proteins in the eggshell of the Siamese crocodile is discussed. For the first time, the presence of porphyrin pigment is reported in a crocodilian eggshell, albeit in a small amount (about 2 to 3 orders of magnitude lower than white avian eggs).
Permanent Link: http://hdl.handle.net/11104/0283659