Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy

0489135 - ÚMCH 2019 RIV US eng J - Journal Article
Pánek, Jiří - Loukotová, Lenka - Hrubý, Martin - Štěpánek, Petr
Distribution of diffusion times determined by fluorescence (lifetime) correlation spectroscopy.
Macromolecules. Roč. 51, č. 8 (2018), s. 2796-2804. ISSN 0024-9297. E-ISSN 1520-5835
R&D Projects: GA MŠMT(CZ) LO1507
Institutional support: RVO:61389013
Keywords : polymer solution * fluorescence correlation spectroscopy * diffusion time distribution
OECD category: Polymer science
Impact factor: 5.997, year: 2018

Fluorescence correlation spectroscopy (FCS) provides most commonly values of diffusion coefficients of fluorescently labeled species in a solution, especially biopolymers. A newly developed procedure for the determination of diffusion coefficient distributions applicable to polydisperse polymers or nanoparticles, based on the well-known CONTIN algorithm, is described and tested on both simulated FCS correlation functions and real experimental data. Good resolution of bimodal distributions is observed, and it is quantitatively established how the resolution depends on the level of experimental noise. Effects of incorrect calibration of the focal volume on the obtained diffusion coefficient and its distribution are described for single-focus FCS. With rapid expansion of the FCS method from biology and biochemistry into the polymer field, and recently into many other disciplines including, e.g., environmental sciences, such a technique was very much needed.
Permanent Link: http://hdl.handle.net/11104/0283730