Coping with iron limitation: a metabolomic study of Synechocystis sp PCC 6803

0489000 - ÚVGZ 2019 RIV PL eng J - Journal Article
Rivas-Ubach, A. - Poret-Peterson, A. T. - Penuelas, J. - Sardans, J. - Pérez-Trujillo, M. - Legido-Quigley, C. - Oravec, Michal - Urban, Otmar - Elser, J. J.
Coping with iron limitation: a metabolomic study of Synechocystis sp PCC 6803.
Acta Physiologiae Plantarum. Roč. 40, č. 2 (2018), č. článku 28. ISSN 0137-5881. E-ISSN 1861-1664
R&D Projects: GA MŠMT(CZ) LO1415; GA MŠMT(CZ) LM2015061
Institutional support: RVO:86652079
Keywords : sp strain pcc-6803 * ocean acidification * unicellular cyanobacterium * marine-phytoplankton * foliar metabolomes * nitrogen-fixation * metal homeostasis * oxidative stress * pacific-ocean * responses * Metabolomics * Metallomics * Iron limitation * Cyanobacteria * Ecological stoichiometry
OECD category: Environmental sciences (social aspects to be 5.7)
Impact factor: 1.608, year: 2018

Iron (Fe) is a key element for all living systems, especially for photosynthetic organisms because of its important role in the photosynthetic electron transport chain. Fe limitation in cyanobacteria leads to several physiological and morphological changes. However, the overall metabolic responses to Fe limitation are still poorly understood. In this study, we integrated elemental, stoichiometric, macromolecular, and metabolomic data to shed light on the responses of Synechocystis sp. PCC 6803, a non-N-2-fixing freshwater cyanobacterium, to Fe limitation. Compared to Synechocystis growing at nutrient replete conditions, Fe-limited cultures had lower growth rates and amounts of chlorophyll a, RNA, RNA: DNA, C, N, and P, and higher ratios of protein: RNA, C: N, C: P, and N: P, in accordance with the growth rate hypothesis which predicts faster growing organisms will have decreased biomass RNA contents and C: P and N: P ratios. Fe-limited Synechocystis had lower amounts Fe, Mn, and Mo, and higher amount of Cu. Several changes in amino acids of cultures growing under Fe limitation suggest nitrogen limitation. In addition, we found substantial increases in stress-related metabolites in Fe-limited cyanobacteria such antioxidants. This study represents an advance in understanding the stoichiometric, macromolecular, and metabolic strategies that cyanobacteria use to cope with Fe limitation. This information, moreover, may further understanding of changes in cyanobacterial functions under scenarios of Fe limitation in aquatic ecosystems.
Permanent Link: http://hdl.handle.net/11104/0283494