Mitochondrion remodeling during T. b. brucei developmental differentiation

0488337 - BC 2018 RIV CZ eng O - Others
Kunzová, M. - Doleželová, Eva - Panicucci, Brian - Zíková, Alena
Mitochondrion remodeling during T. b. brucei developmental differentiation.
2017
R&D Projects: GA MŠMT LL1205
Institutional support: RVO:60077344
Keywords : Mitochondrion remodeling * OXPHOS * T. brucei
OECD category: Biochemistry and molecular biology

Trypanosoma brucei undergoes a complex life cycle as it alternates between a mammalian host and the blood-feeding insect vector, a tsetse fly. Due to the different environments, the distinct life stages differ in their energy metabolism, i.e. insect stage (procyclic cells, PS) depends on mitochondrial oxidative phosphorylation (OXPHOS) for ATP production while the bloodstream stage (BS) gains energy by aerobic glycolysis. The dramatic switch from the OXPHOS to glycolysis happens during the complex development of the PS in the tsetse fly. This development differentiation is characterized by extensive remodeling of mitochondrion structure and changes in mitochondrial bioenergetics. Importantly, the molecular mechanism behind this process is completely unknown. We have established the in vitro differentiation system, in which the transition from PS to epimastigotes followed by differentiation to transmission-ready metacylic trypanosomes is triggered by RNA binding protein 6 (RBP6) expression. This in vitro induced differentiation of PF cells takes 8 days. The appearance of epimastigotes and metacyclic trypanosomes in the culture was mapped using light and fluorescent microscopy. The whole cell proteome of cell culture harvested every day after the RBP6 induction was identified by label-free quantitative mass spectrometry. This proteomic data serves as a resource for further detailed characterization of changes happening in the parasite mitochondrion as well as identification of possible candidates involved in the PS differentiation.
Permanent Link: http://hdl.handle.net/11104/0282922