Recovery from supercooling, freezing, and cryopreservation stress in larvae of the drosophilid fly, Chymomyza costata

0488068 - BC 2019 RIV GB eng J - Journal Article
Štětina, Tomáš - Hůla, Petr - Moos, Martin - Šimek, Petr - Šmilauer, P. - Košťál, Vladimír
Recovery from supercooling, freezing, and cryopreservation stress in larvae of the drosophilid fly, Chymomyza costata.
Scientific Reports. Roč. 8, MAR 13 (2018), č. článku 4414. ISSN 2045-2322. E-ISSN 2045-2322
R&D Projects: GA ČR(CZ) GA16-06374S
Institutional support: RVO:60077344
Keywords : insect * cold tolerance * cryopreservation
OECD category: Biology (theoretical, mathematical, thermal, cryobiology, biological rhythm), Evolutionary biology
Impact factor: 4.011, year: 2018
http://www.nature.com/articles/s41598-018-22757-0

Here we report on recovery of drosophilid fly larvae (Chymomyza costata)//from three different levels of cold stress: supercooling to -10°C, freezing at -30°C, and cryopreservation at -196°C. We found that recovery from all three cold stresses requires access to additional energy reserves to support cold-injury repair processes. Metabolomic profiling and custom microarray analysis indicated that additional energy was needed to: clear by-products of anaerobic metabolism, deal with oxidative stress, //re-fold partially denatured proteins, and remove damaged proteins, complexes and/or organelles. Majority of cryopreseved larvae failed to proceed in ontogenesis, showed specific metabolic perturbations suggesting impaired mitochondrial function, and failed to up-regulate a set of 116 specific genes potentially linked to repair of cold injury.
Permanent Link: http://hdl.handle.net/11104/0284084