Development, characterization and cross-amplification of 16 microsatellite primers for Atriplex tatarica (Amaranthaceae)

0487739 - BÚ 2018 RIV US eng J - Journal Article
Kondrysová, E. - Krak, Karol - Mandák, Bohumil
Development, characterization and cross-amplification of 16 microsatellite primers for Atriplex tatarica (Amaranthaceae).
Applications in Plant Sciences. Roč. 5, č. 11 (2017), s. 1-5, č. článku 1700094. ISSN 2168-0450. E-ISSN 2168-0450
Institutional support: RVO:67985939
Keywords : Amaranthaceae * Atriplex * Chenopodium
OECD category: Plant sciences, botany
Impact factor: 1.187, year: 2017

Sixteen novel microsatellite primers were developed for A. tatarica based on high-throughput sequencing of enriched libraries. All markers were polymorphic, with the number of alleles per locus ranging from three to 25 and observed and expected heterozygosity ranging from 0.08 to 0.74 and 0.10 to 0.87, respectively. In addition, some of these loci were successfully amplified and showed polymorphisms in four Atriplex and seven Chenopodium species. The microsatellite markers published here will be useful in assessing genetic diversity, structure, and gene flow within and across populations of A. tatarica, as well as in other species of Atriplex and the related genus Chenopodium.
Permanent Link: http://hdl.handle.net/11104/0282373