Aerobic Anoxygenic Photosynthesis Is Commonly Present within the Genus Limnohabitans

0487086 - BC 2018 RIV US eng J - Journal Article
Kasalický, Vojtěch - Zeng, Yonghui - Piwosz, Kasia - Šimek, Karel - Kratochvílová, Hana - Koblížek, Michal
Aerobic Anoxygenic Photosynthesis Is Commonly Present within the Genus Limnohabitans.
Applied and Environmental Microbiology. Roč. 84, č. 1 (2018), č. článku e02116-17. ISSN 0099-2240. E-ISSN 1098-5336
R&D Projects: GA MŠMT(CZ) EE2.3.30.0032; GA ČR(CZ) GA13-00243S; GA ČR GA15-12197S; GA MŠMT(CZ) LO1416
Institutional support: RVO:60077344 ; RVO:61388971
Keywords : fish * IR microscopy * Limnohabitans * bacteriochlorophyll * bchY
OECD category: Microbiology; Microbiology (MBU-M)
Impact factor: 4.077, year: 2018

The genus Limnohabitans (Comamonadaceae, Betaproteobacteria) is a common and a highly active component of freshwater bacterioplanktonic communities. To date, the genus has been considered to contain only heterotrophic species. In this study, we detected the photosynthesis genes pufLM and bchY in 28 of 46 strains from three Limnohabitans lineages. The pufM sequences obtained are very closely related to environmental pufM sequences detected in various freshwater habitats, indicating the ubiquity and potential importance of photoheterotrophic Limnohabitans in nature. Additionally, we sequenced and analyzed the genomes of 5 potentially photoheterotrophic Limnohabitans strains, to gain further insights into their phototrophic capacity. The structure of the photosynthesis gene cluster turned out to be highly conserved within the genus Limnohabitans and also among all potentially photosynthetic Betaproteobacteria strains. The expression of photosynthetic complexes was detected in a culture of Limnohabitans planktonicus II-D5T using spectroscopic and pigment analyses. This was further verified by a novel combination of infrared microscopy and fluorescent in situ hybridization.
Permanent Link: http://hdl.handle.net/11104/0281771